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Fig. 1. Component selection for efficient in vitro differentiation of NPCs into neurons. Immunostaining of mouse NPCs at day 14 of differentiation. Neurons were determined by the expression of TUJ1 and SYNAPSIN1. AA- and B27-treated NPCs were used as the negative control, and the NPCs treated with a cytokine mixture (AA, BDNF, GDNF, DX, cAMP, B27) were used as the positive control. (A) Each negative control was respectively added, and the differentiation-related cytokines (BDNF, GDNF, DX, cAMP) were evaluated. (B) For the positive control, each differentiation-related cytokine (BDNF, GDNF, DX, cAMP) was respectively eliminated. (C) Counts of TUJ1+ or SYNAPSIN1+ neuronal cells at 14 days post-induction with differentiation-related cytokines are shown in panel A. (D) Counts of TUJ1+ or SYNAPSIN1+ neuronal cells at 14 days post-induction with differentiation-related cytokines are shown in panel B. Error bars, SE. *p<0.05, Scale bar, 50 μm.
Biomolecules & Therapeutics 2019;27:78~84 https://doi.org/10.4062/biomolther.2018.110
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