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Fig. 5. LPS stimulation induces the PD-1 expression on MDSCs. The CD11b+ cells were cultured in fresh medium in the presence of 10 ng/ml GM-CSF and then pre-treated with PmB (100U). Next, LPS (A, B) or TCCM (C, D) was treated in cells. (E, F) The CD11b+ cells were cultured in fresh medium in the presence of 10 ng/ml GM-CSF, in the absence or presence of LPS and TCCM. The cells were collected on day 4 for cell analysis. The experiments were independently performed at least 3 times.
Biomolecules & Therapeutics 2019;27:63~70 https://doi.org/10.4062/biomolther.2018.201
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