Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 2. Tumor microenvironment induces PD-1 expression on MDSCs. Bone marrow cells were obtained from the femurs of Balb/c mice, and then CD11b+ cells were sorted. (A, B) The CD11b+ cells were cultured in fresh medium in the presence of 10 ng/ml GM-CSF, in the absence or presence of IL-6 or TCCM. The cells were collected on day 4 for cell analysis. (C, D) The CD11b+ cells were cultured in fresh medium in the presence of 10 ng/ml GM-CSF, in the absence or presence of indicated concentrations of TCCM. The cells were collected at 24 h and 96 h for cell analysis. (E, F) BM-MDSCs were co-cultured with 4T1 tumor cells (ratio of 10:1). After 96 h, non-adherent cells were collected for cell analysis. All experiments were independently repeated at least 3 times and a representative figure was shown in (A), (C), and (E). **p<0.01, ***p<0.001.
Biomolecules & Therapeutics 2019;27:63~70 https://doi.org/10.4062/biomolther.2018.201
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