Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 4. TGase 2 inhibition or TGase 2 knock down reversed rapamycin resistance in cancer cells. (A) TGase 2 inhibition reversed rapamycin induced autophagy in cancer cells which potentiated anti-cancer effect with rapamycin. CAKI-1 and A549 cell were treated with GK921 or Rapamycin and combination for 24h with chloroquine (50 µM) for 6 h under amino acid deprivation for 4 h. (B) TGase 2 knock down reversed rapamycin induced autophagy in cancer cells which potentiated anti-cancer effect with rapamycin. Under the same condition of (A), these cells were treated with TGase 2 siRNA or rapamycin and combination of TGase 2 siRNA and rapamycin. (C) XTT assay showed that TGase 2 inhibition reversed rapamycin resistance. Rapamycin resistant cancer cells of MCF7RR and HCT116RR were cultured with Rapamycin (20 µM). The cells were treated with TGase 2 inhibitor GK921, rapamycin and combination of GK921 and rapamycin for 24 h. (D) XTT assay showed that TGase 2 knock down reversed rapamycin resistance. Rapamycin resistant cancer cells of MCF7RR and HCT116RR were treated with siRNA of TGase 2, rapamycin and combination of siRNA of TGase 2 and rapamycin for 24 h. Cumulative data from three independent experiments is shown here as mean ± SD (n=3). **p<0.01, ***p<0.001. e="" tgase="" 2="" was="" increased="" in="" the="" drug="" resistant="" cancer="" cells="" analyzed="" by="" immunoblotting="" of="" tgase="" 2="" rapamycin="" resistant="" cancer="" cells="" such="" as="" mcf7="" sup="">RR and HCT116RR showed higher expression level of TGase 2 to compare to rapamycin sensitive cancer cells such as MCF7 and HCT116. (F) Summary of TGase 2 mediated regulation of autophagy.
Biomolecules & Therapeutics 2019;27:34~40 https://doi.org/10.4062/biomolther.2018.140
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