Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 3. LC transcription is suppressed by p53 in cancer cells. (A) p53 transfection suppressed LC3 expression. CAKI-1 and A549 cells were transfected with 3xFLAG p53 (p53 over-expression vectors: 0, 1, 2, 4 µg) for 24 h, and treated with chloroquine (50 µM) for 6 h under amino acid deprivation for 4 h. qRT-PCRs against p53, LC3 and β-actin were performed as described in the methods. (B) p53 knock down increased LC3 expression. MCF7 and HCT116 cells were transfected with siRNA of p53 (0, 20, 40 nM) for 24 h, and treated with chloroquine (50 µM) for 6 h. Real-time PCR against p53, LC3 and β-actin were performed as described in the methods. (C) TGase 2 knock down decreased LC3 expression. CAKI-1 and A549 cells were transfected with TGase 2 siRNA for 24 h in a dose dependent manner (0, 10, 20, 40 nM) (D) TGase 2 transfection increased LC3 expression. MCF7 and HCT116 cells were transfected with HA-TGase2 in a dose dependent manner (0, 1, 2, 4 µg). (E) TGase 2 knock down or inhibition increased p53 level. CAKI-1 and A549 cells were transfected with siRNA of TGase 2 or treated with GK921 (1 µM). (F) TGase 2 transfection decreased p53, which was reversed by TGase 2 inhibition. MCF7 and HCT116 cells were transfected with HA-TGase 2 plasmid alone or combined with GK921 (1 µM).
Biomolecules & Therapeutics 2019;27:34~40 https://doi.org/10.4062/biomolther.2018.140
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