Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

Download original image
Fig. 2. Inhibition of TGase 2 induces p53 stability. (A) Before harvest the samples, CAKI-1 and A549 cells were treated with GK921 (0, 250, 500, 1000 nM) for 8h, overlapped with chloroquine treatment (CQ, 50 µM) for 6 h under amino acid deprivation for 4 h. Whole-cell lysates were subjected to the immunoblotting with indicated antibodies. (B) Under the same condition as above, cells were stained with CYTPO-ID green detection reagent for autophagy activity. (C) Cells with the low level of TGase 2 such as HCT116 and MCF7 or TGase 2 transfected cells such as HCT116TG2 and MCF7TG2 cells were treated with GK921 (0, 250, 500, 1000 nM) for 8h, under the same condition as above. Whole-cell lysates were subjected to immunoblotting of TGase 2 as well as measuring autophagy activity using CYTO-ID green detection reagent. Cumulative data from three independent experiments is shown here as mean ± SD (n=3). ***p<0.001.
Biomolecules & Therapeutics 2019;27:34~40 https://doi.org/10.4062/biomolther.2018.140
© Biomolecules & Therapeutics